Win 10 and Chrome on 2 different computers, I no longer lose them when I refresh , but when I move from one system to the other (Home/Work) many times the hot keys are lost.
Once again, hotkeys on not saved on tw2, they are saved on ur computer in the cookie file of tw2
Clear cookies delete them, or from what i understand playing multiple words MAY clear them as well ( i am not 100% sure onn this as i have never tested that)